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    Home / Rapid Diagnostic Tests / Sickle Cell Disease
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    ASI Sickle Cell Test

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    Categories: Rapid Diagnostic Tests, Sickle Cell Disease
    • Description
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    The ASI Sickle Cell Test is a convenient and specific kit intended to be used as an aid in the qualitative detection of hemoglobin S (Hb-S) in anticoagulated whole blood. The test does not distinguish between sickle cell disease (HbS/S) and sickle cell trait (HbS/A). This test is not recommended for use on newborns under 3 months of age. Kit does not include controls.

    Features

    • Accurate, easy and economical
    • Detects both homozygous (S/S) and heterozygous (A/S) -sickle cell
    • Contains urea reagent for test confirmation
    • Turbidity line test based upon modified Nalbandian procedure
    • Room temperature storage
    • Longest shelf life – up to 18 Months
    • Proudly made in USA

    Sensitivity & Specificity

    Sensitivity: >99%
    Specificity: >99%

    Certifications

    CPT Code 85660
    510(k)-K960947

     


    Sickle Cell Procedures and Interpretation

    Preparation of working sickle cell buffer

    Add the entire contents of one vial of Sickle Cell Lysing Reagent to 50ml of Sickle Cell Buffer and mix completely by swirling. The working buffer may be used for 30 days from date of preparation if stored at 2° to 8° C.

    Assay Protocol

    1. Transfer 2.0ml of the Working Sickle Cell Buffer to a 12 x 75 mm tube.
    2. Add 20μl of well-mixed whole blood or control specimen and mix thoroughly and gently. If testing a specimen with a hemoglobin below 7 g/dl, add 40 μl of whole blood.
    3. Allow to stand for 5 minutes but no longer than 30 minutes at room temperature (15° to 30° C).
    4. Read results by looking through the test tube at the Line Scale (see below). Hold the test tube upright and perpendicular approximately 3 cm from the Line Scale. Adequate illumination is necessary.

    Interpretation

    Reactive – If any sickling hemoglobin is present, the solution will be sufficiently turbid to prevent reading the Line Scale through the test tube.

    Nonreactive – If a sickling hemoglobin is not present, the solution will be clear enough to allow the Line Scale to be seen through the test tube.

    Read results by looking through the test tube at the line scale holding the test tube upright approximately 3 cm from line scale.

    Reactive will be turbid to prevent reading the line scale. Nonreactive, the solution will be clear and line scale can be seen through test tube.

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    About us

    Lighthouse Medical Supplies Ltd. is a Bermuda-based medical, long term care, surgical, dental and laboratory supply and equipment provider.

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    • Clinical
      • Medical furniture
        • Examination tables
      • Central sterile
        • Steam sterilizers
      • Clinical diagnostics
        • Physical examination
        • Respiratory monitoring
        • Cardiac monitoring
        • Spot check vital signs
        • ENT and ophthalmic instruments
      • Medical Imaging
        • MRI patient monitors
        • Radiography suites
        • Ultrasound scanners
        • Ultrasound probe disinfector
        • Beekley imaging & radiation therapy markers
      • Emergency / Trauma
        • AEDs
      • Consumables
        • IV Catheters
    • Dental
      • Rotary
    • Laboratory
      • Analyzers
        • Hematology
        • Clinical chemistry
        • Hemostasis
        • Urinalysis
      • Laboratory equipment
        • Biosafety
        • Centrifuges
        • Microscopes
      • Medical laboratory consumables
        • Microbiology consumables
        • Nichrome Loops
      • Rapid Diagnostic Tests
        • Sickle Cell Disease
        • Bacterial vaginosis
        • Herpes (HSV-2)
        • Mononucleosis
        • Staph aureus
        • Hemoglobin
        • Syphilis
    • Facilities management
      • Medical interiors and furniture
      • Air purifiers
    • Services
    • Blog
    • Contact
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    • Careers
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